Efficiency enhancement of long-range energy transfer by seqencial multistep FRET using fluorescence labeled DNA
نویسندگان
چکیده
منابع مشابه
Fluorescence resonance energy transfer (FRET) sensors
The principle of "fluorescence resonance energy transfer" (FRET) has been exploited in cell biology to demonstrate the interaction of two proteins in living cells. FRET is highly specific and sensitive and allows for the online recording of events that modulate the interaction. We here demonstrate that FRET-based sensor molecules represent an alternative in monitoring receptor-dependent signall...
متن کاملFluorescence Resonance Energy Transfer (FRET) sensor
The FRET has been widely used as a spectroscopic technique in all applications of fluorescence including medical diagnostics, DNA analysis, optical imaging [1] and for various sensing properties [2-11]. Generally, fluorescence-based sensors adopt three different strategies: (a) fluorescence quenching (turn-off), (b) fluorescence enhancement (turn-on) and (c) ratiometric FRET. FRET sensors becam...
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DNA is a unique molecule that can be used to separate donor and acceptor for fluorescence energy transfer (ET)1 and longrange photoinduced electron-transfer studies.2 ET has been used extensively as a spectroscopic ruler for biological structures,3a-c and ET primers and terminators are markedly superior to single dye-labeled reagents in DNA sequencing and analysis.4a-b Molecular beacons using b...
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Fluorescence resonance energy transfer (FRET) is an extremely effective tool to detect molecular interaction at suboptical resolutions. One of the techniques for measuring FRET is acceptor photobleaching: the increase in donor fluorescence after complete acceptor photobleaching is a measure of the FRET efficiency. However, in wide-field microscopy, complete acceptor photobleaching is difficult ...
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Abbreviations: CPRG, chlorophenol red-β-D-galactopyranoside; CFP, Cyan Fluorescent Protein; CHO, Chinese Hamster Ovary cells; FRET, Fluorescence Resonance Energy Transfer; GFP, Green Fluorescent Protein; HEX, hexachloro-carboxyfluorescein; HUVEC, Human Umbilical Vein Endothelial Cells; RHD, Rel Homology Domain; TET, tetrachloro-carboxyfluorescein; β-TrCP, beta-transducin repeat-containing prote...
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ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 1999
ISSN: 0261-3166,1746-8272
DOI: 10.1093/nass/42.1.241